fig5

Figure 5. Alteration of dopamine content and release in the striatum of G59S Tg and G71R Tg mice. (A) HPLC measures the content of dopamine and DOPAC in the dorsal striatum of 18-month-old nTg, WT Tg, G59S Tg, and G71R Tg mice (n = 3 or 4 per genotype). Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test was used for statistical analysis (the mean of each genotype was compared with the mean of nTg). ^*P = 0.0397. ns: Not significant. (B, C) FSCV quantifies the peak evoked dopamine release (B) and time constant of slope decay (C) following single-pulse electrical stimulation of different stimulus intensity in the dorsal striatum of six-month-old nTg, WT Tg, G59S Tg, and G71R Tg mice (n = 3 animals per genotype and 3 sections per animal). Data are presented as mean ± SEM. Two-way ANOVA genotype factor: nTg vs. G59S Tg, F_(1,12) = 25.11, ^**P = 0.0044; nTg vs. G71R Tg, F_(1,12) = 40.19, ^****P < 0.0001 (B). Two-way ANOVA genotype factor: nTg vs. G59S Tg, F_(1,12) = 9.285, ^*P = 0.0101; nTg vs. G71R Tg, F_(1,12) = 8.491, ^*P = 0.0130 (C). (D,E) FSCV quantifies the peak evoked dopamine release (D) and time constant of slope decay (E) in response to burst electrical stimulation (200 μA, 50 Hz, 5 pulses) in the dorsal striatum of 18-month-old nTg, WT Tg, G59S Tg, and G71R Tg mice (n = 4 or 5 animals per genotype and 3 sections per animal). Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test was used for statistical analysis (the mean of each genotype was compared with the mean of nTg). nTg vs. G59S Tg, ^***P = 0.0004; nTg vs. G71R Tg, ^**P = 0.0034 (D). nTg vs. G59S Tg, ^****P < 0.0001; nTg vs. G71R Tg, ^*P = 0.0357 (E).